Plasmid

Part:BBa_K4258060:Design

Designed by: Jake Schumacher   Group: iGEM22_NDSU   (2022-10-09)


J23102+BCD2+CDS#20+B0015+DVK


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 143
    Illegal BsaI.rc site found at 873


Design Notes

All parts were designed to be Golden Gate MoClo compatible with Bsa1 sites.


Source

BBa_J23102 BBa_K1114107 BBa_K4258020 BBa_B0015

References