Plasmid
Part:BBa_K4258060:Design
Designed by: Jake Schumacher Group: iGEM22_NDSU (2022-10-09)
J23102+BCD2+CDS#20+B0015+DVK
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 143
Illegal BsaI.rc site found at 873
Design Notes
All parts were designed to be Golden Gate MoClo compatible with Bsa1 sites.
Source
BBa_J23102 BBa_K1114107 BBa_K4258020 BBa_B0015